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mRNA vaccine–induced neoantigen-specific T cell immunity ...

Phorbol 12-myristate 13-acetate–ionomycin mixture (eBioscience) was used as a positive control. IFN-γ ELISPOT assays were performed on MultiScreen-IP filter plates (MilliporeSigma). Each plate was pretreated with 50 μL 70% ethanol/well for less than 2 minutes, washed 4 times with ultra-pure water (Quality Biological), and then coated with 10 μg/mL IFN-γ capture antibody (100 μl/well ...

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The aim of CGMPs is to provide a framework to ensure high-quality production in well-controlled facilities and equipment by well-trained and regularly trained staff, while requiring a wide range of systems that document all aspects of operation in order to demonstrate continuous compliance. The quality control of CAR-T cell products should include a full range of tests on quality, safety, and ...

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